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Millipore

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

sample volume 2 mL, regenerated cellulose membrane, MWCO 10 kDa

Synonym(s):

Centrifugal concentrator, 10 kDa MWCO, 2 mL sample volume

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About This Item

UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24

material

polypropylene tube (for collection)
regenerated cellulose membrane
styrene-butadiene housing

Quality Level

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Ultra

parameter

2 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

L

119.7 mm

diam.

15.9 mm

filtration area

1 cm2

volume

2 mL

pore size

10 kDa MWCO

shipped in

ambient

General description

Amicon® Ultra-2 centrifugal filter devices offer fast ultrafiltration. It has the potential to recover high concentration factors and easy concentrate from dilute and complex sample matrices. Amicon® Ultra-2 devices are supplied non-sterile and are for single use only.

Application

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates.
  • Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.
Amicon® Ultra-2 Centrifugal Filter Unit has been used:
  • to reduce large volume biofluids prior to extracellular vesicle (EV) isolation or to concentrate EVs
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids (DNA/RNA samples, either single- or double-stranded), microorganisms, column eluates, and purified samples
  • Purification of macromolecular components found in tissue culture extracts and cell lysates, removal of primer, linkers, or molecular labels from a reaction mix, and protein removal before high-performance liquid chromatography (HPLC)
  • Desalting, buffer exchange, or diafiltration

Features and Benefits

  • Amicon® Ultra 2ml 10K 24PK
  • Concentrates 2 mL down to 15-70 μl
  • 98% sample recovery
  • Fast processing time - 10 to 30 minute spin times
  • Engineered dead stop
  • The reverse spin capability provides consistent recoveries

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 2 mL down to 15-70 μL
  • 98% sample recovery
  • Fast processing time: 10 to 30-minute spin times
  • Engineered dead stop
  • Reverse spin capability provides consistent recoveries

Linkage

Replaces: 4205

Other Notes

The Amicon® Ultra-2 device is supplied with two tubes. During operation, one tube is used to collect filtrate; the other to cap the device during concentration and subsequently to recover the concentrated sample. Amicon Ultra-2 Centrifugal filter devices are for research use only and not for use in diagnostic procedures.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Bao Quoc Tran et al.
Data in brief, 6, 68-76 (2016-01-23)
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated

Articles

This page discusses the principles and benefits of using ultrafiltration membranes in protein sample preparation. Possible applications include solvent exchange, protein sample concentration, or sample purification.

Related Content

Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

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