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10165875001

Roche

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

from Leuconostoc mesenteroides

Synonym(s):

G6P-DH, Glucose-6-Phosephate Dehydrogenase

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About This Item

Enzyme Commission number:
1.1.1.49 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

bacterial (Leuconostoc mesenteroides)

Quality Level

100

form

solution
suspension

specific activity

~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)

mol wt

dimer 110 kDa

packaging

pkg of 1 mL (1,000 U)

manufacturer/tradename

Roche

concentration

≥0.1-1.0 % (w/w)

technique(s)

activity assay: suitable

color

white

optimum pH

7.0-8.5(maximal activity at 7.8)

solubility

water: miscible

NCBI accession no.

AAA25265

UniProt accession no.

P11411

application(s)

life science and biopharma

foreign activity

6-PGDH <0.001%
CK <0.001%
GR <0.01%
HK <0.05%
NADH oxidase <0.02%
PGI <0.01%

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1

Specificity

Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (Km = 36 μM, NADP as coenzyme; 64 μM, NAD as coenzyme), but will use either NADP (Km = 7.4 μM; relative rate 1.0) or NAD (Km = 115 μM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.

Application

Component of cofactor recycling systems for NADPH.
It was used in enzymatic determination of C6 phosphorylation of glycogen.

Quality

Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH

Unit Definition

Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme

Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.

Physical form

Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6

Preparation Note

Activator: HCO3- (≤ 0.3 M) activates slightly.

Analysis Note

Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Certificates of Analysis (COA)

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Anna A DePaoli-Roach et al.
The Journal of biological chemistry, 290(2), 841-850 (2014-11-25)
Glycogen is a branched polymer of glucose that acts as an energy reserve in many cell types. Glycogen contains trace amounts of covalent phosphate, in the range of 1 phosphate per 500-2000 glucose residues depending on the source. The function

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