Skip to Content
MilliporeSigma
All Photos(1)

Documents

G5760

Sigma-Aldrich

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

Synonym(s):

G-6-P-DH

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9001-40-5
Enzyme Commission number:
1.1.1.49 (BRENDA, IUBMB)
EC Number:
232-602-6
MDL number:
MFCD00081656
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Leuconostoc mesenteroides)

Quality Level

200

type

Type XXIII

form

ammonium sulfate suspension

specific activity

550-1,100 units/mg protein (biuret)

mol wt

54 kDa

concentration

≥2.0 mg/mL Biuret

foreign activity

6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%
PGI ≤0.01%

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+). It corresponds to a molecular weight of 54 kDa and exists as a homodimer.

Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:
  • as a component of reaction mixture for assaying mannose- and glucose-6-phosphate activity
  • as a component of reaction mixture in the nevirapine inhibition studies in human hepatic microsomes
  • in the glucose-phosphorylating activity of chloroplast extracts

Glucose-6-phosphate dehydrogenase was used as a model to test the effect of seed protein fractions on enzyme protection during dehydration. G-6-PDH has been utilized in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.
Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD+. G6PD uses NAD+ or NADP+ based on the catabolic or anabolic metabolic pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Suspension in 2.7 M (NH4)2SO4 solution containing 42 mM Tris and 0.8 mM MgCl2

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A novel type of chloroplast stromal hexokinase is the major glucose-phosphorylating enzyme in the moss Physcomitrella patens
Olsson T, et al.
The Journal of Biological Chemistry, 278(45), 44439-44447 (2003)
In vitro studies: inhibition of nevirapine metabolism by nortriptyline in hepatic microsomes
Usach I and Peris JE
Bio-protocol, 5(19) (2014)
Oligomerization studies of Leuconostoc mesenteroides G6PD activity after SDS-PAGE and blotting
Ravera S, et al.
Molecular Biology, 44(3), 415-419 (2010)
Lysine-21 of Leuconostoc mesenteroides glucose 6-phosphate dehydrogenase participates in substrate binding through charge-charge interaction
Lee WT and Levy HR
Protein Science, 1(3), 329-334 (1992)
A Modified Method for Detection of Post-Phoretic Activities in Mannose-(Mpi, EC 5.3. 1.8) and Glucose-(Gpi, EC 5.3. 1.9) 6-Phosphate Isomerases
Zasypkin MY, et al.
Russian Journal of Genetics, 37(5), 581-583 (2001)
View All Related Papers

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service