PEFBSC-RO
Roche
Pefabloc® SC
powder, solubility: 100 mg/mL in aqueous buffer, suitable for blocking, suitable for protein purification
Synonym(s):
Pefabloc® SC, 4-(2-aminoethyl)-benzene-sulfonyl fluoride, aebsf, aminoethyl-benzene-sulfonyl fluoride, 4-2-, proteinase k inhibitor
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
12352200
Recommended Products
form
powder
Quality Level
mol wt
Mr 239.5
packaging
pkg of 1 g (11429876001)
pkg of 100 mg (11429868001)
pkg of 500 mg (11585916001)
manufacturer/tradename
Roche
technique(s)
blocking: suitable
protein purification: suitable
mp
175-185 °C
solubility
aqueous buffer: 100 mg/mL
storage temp.
2-8°C
Related Categories
General description
Pefabloc SC is a specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity is comparable to PMSF or DFP, but Pefabloc SC is non-toxic.
Specificity
Pefabloc SC belongs to the family of sulfonyl fluorides which irreversibly block serine proteases. Pefabloc SC was also described as potent serine threonine phosphatase inhibitor.
Application
- Pefabloc SC is used to inhibit the detrimental effects of proteases during preparative protein purification.
- Due to its low toxicity toward eukaryotic cells, it may be applied in the production of recombinant proteins, during fermentation of transformed cells, where proteolytic digestion may decrease the yield of the desired product.
- Pefabloc SC is used to completely inactivate proteinase K during the preparation of chromosomal DNA in agarose plugs. For this purpose, the cells are embedded in agarose and digested with proteinase K to degrade all proteins. Before a specific restriction endonuclease is added, the proteinase K is inactivated by incubating the agarose plugs in 1 to 5 mM Pefabloc SC in 10 mM Tris-HCl, 1 mM EDTA , pH 7 for 2 hours at +37 °C or overnight.
- In contrast to PMSF, Pefabloc SC is an excellent blocker of thrombin activity in serum or plasma. In these biological fluids, PMSF interacts in a reversible manner with albumin, which reduces its free concentration and leads to a delay in thrombin inactivation. Pefabloc SC, however, does not react with serum albumin, and exhibits a threefold higher capacity to inactivate thrombin under similar conditions.
- Pefabloc SC can be used on living cells.
- Use Pefabloc SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE).With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis.
Features and Benefits
- Benefit from an easy-to-use inhibitor. Add water-soluble Pefabloc SC directly to aqueous buffers.
- Avoid hazardous compounds. Obtain non-toxic protease inhibition without risk to you, or those around you (see Figure 1).
- Ensure protection with improved stability. Achieve consistent protease inhibition even at pH levels above 7.0 and temperatures above +4°C (see Figure 2).
- Maximize inhibition. Be certain that your levels of active inhibitor are high enough by using a product that is reliably soluble and stable.
Quality
Function tested; homogeneous in TLC
Preparation Note
Working concentration: 0.1 to 1 mg/ml (0.4 to 4 mM)
Working solution: Solvent is recommended in aqueous buffers or distilled water up to 100 mg/ml.
Storage conditions (working solution): -15 to -25 °C (stock solution)
The stability of Pefabloc SC in aqueous solution is affected both by pH and temperature. Concentrated stock solutions (e.g., 100 mM) prepared in dist. water are acidic and stable for 1 to 2 months at -15 to -25 °C, if stored in aliquots.
Note: Store Pefabloc SC under acidic conditions and add to biological samples shortly before use to minimize hydrolysis.
Working solution: Solvent is recommended in aqueous buffers or distilled water up to 100 mg/ml.
Storage conditions (working solution): -15 to -25 °C (stock solution)
The stability of Pefabloc SC in aqueous solution is affected both by pH and temperature. Concentrated stock solutions (e.g., 100 mM) prepared in dist. water are acidic and stable for 1 to 2 months at -15 to -25 °C, if stored in aliquots.
Note: Store Pefabloc SC under acidic conditions and add to biological samples shortly before use to minimize hydrolysis.
Reconstitution
Soluble up to 100 mg/ml in aqueous buffers or water. Stable in solution for 1 to 2 months if stored in aliquots at -15 to -25 °C. Only slight hydrolysis occurs under weakly basic conditions (pH 8.0 to 9.0).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
Pefabloc is a registered trademark of Pentapharm
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class
8A - Combustible, corrosive hazardous materials
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Roxanne C S van Adrichem et al.
Endocrine connections, 5(4), 143-151 (2016-05-25)
To date, the value of fasting plasma acylated ghrelin (AG) and unacylated ghrelin (UAG) as potential novel biomarkers in patients with neuroendocrine tumors (NETs) is unknown. The aims of this study are to (i) compare fasting AG and UAG levels
Jae Wook Hyeon et al.
Scientific reports, 5, 14944-14944 (2015-10-10)
Prion diseases are associated with the conformational conversion of the physiological form of cellular prion protein (PrP(C)) to the pathogenic form, PrP(Sc). Compounds that inhibit this process by blocking conversion to the PrP(Sc) could provide useful anti-prion therapies. However, no
Theresa J Barberi et al.
PloS one, 7(1), e29808-e29808 (2012-01-13)
Pin1 has previously been described to regulate cells that participate in both innate and adaptive immunity. Thus far, however, no role for Pin1 has been described in modulating conventional dendritic cells, innate antigen presenting cells that potently activate naïve T
Patric J D Delhanty et al.
Clinical endocrinology, 82(1), 142-146 (2014-05-09)
The acylated/unacylated ghrelin (AG/UAG) ratio has been reported to range from 0·02 to 0·3, suggesting biologically relevant independent regulation of each ghrelin isoform. However, AG is deacylated to UAG by esterases in blood samples, and esterase inhibition is critical for
Huiwen Song et al.
Autophagy, 11(8), 1308-1325 (2015-06-18)
Recent studies have shown that the phosphorylation and dephosphorylation of ULK1 and ATG13 are related to autophagy activity. Although ATG16L1 is absolutely required for autophagy induction by affecting the formation of autophagosomes, the post-translational modification of ATG16L1 remains elusive. Here
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service