Skip to Content
MilliporeSigma
All Photos(1)

Documents

76307

Supelco

Pefabloc® SC

analytical standard

Synonym(s):

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, AEBSF

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C8H10FNO2S · HCl
CAS Number:
30827-99-7
Molecular Weight:
239.69
Beilstein/REAXYS Number:
7604627
MDL number:
MFCD00132962
UNSPSC Code:
41116107
PubChem Substance ID:
329767025
NACRES:
NA.24

grade

analytical standard

Quality Level

200

assay

≥95.0% (HPLC)

form

powder or crystals

shelf life

5 yr

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

forensics and toxicology
pharmaceutical (small molecule)

format

neat

storage temp.

2-8°C

SMILES string

FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl

InChI

1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H

InChI key

WRDABNWSWOHGMS-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Pefabloc® SC is a potent, nontoxic and relatively easy to use, irreversible serine proteinase inhibitor. It is reported to efficiently inhibit the platelet activating factor (PAF)-degrading acetylhydrolase (acetylhydrolase) in both humans and rats.
Pefabloc® SC is a trypsin inhibitor, which can be used in the synthesis of its arginine-like analogue, 4-(2-guanidinoethyl)benzenesulfonyl fluoride.

Application

Pefabloc® SC may be used as an analytical standard in determining the native oligomeric state and substrate specificity of rat NTPDase1 and NTPDase2 after heterologous expression in Xenopus oocytes. It may also be used as an esterase inhibitor, which is added to the whole blood to improve the stability of ciclesonide for the determination of ciclesonide (CIC) and its active metabolite, desisobutyryl-ciclesonide (des-CIC), in human plasma using liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometric (LC-APCI-MS/MS) method.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Recommended concentrations for use are 0.1-1 mM.

Biochem/physiol Actions

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.

Other Notes

Potent and irreversible inhibitor of serine proteases; in contrast to PMSF, it is water soluble and non-toxic; Unaffected by albumin

Legal Information

Pefabloc is a registered trademark of Pentapharm

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 5

1 of 5

Trypsin-chymotrypsin inhibitor from Glycine max (soybean) lyophilized powder

Sigma-Aldrich

T9777

Trypsin-chymotrypsin inhibitor from Glycine max (soybean)

PMSF Phenylmethylsulfonyl fluoride

Roche

PMSF-RO

PMSF

Leupeptin microbial, ≥90% (HPLC)

Sigma-Aldrich

L2884

Leupeptin

4-Bromophenylboronic acid ≥95.0%

Sigma-Aldrich

B75956

4-Bromophenylboronic acid

Pepstatin A microbial, ≥75% (HPLC)

Sigma-Aldrich

P4265

Pepstatin A

Z B Salama
Acta biologica et medica Germanica, 39(4), 355-366 (1980-01-01)
New test systems using azocasein or double-labelled cytosol proteins from rat livers were developed to test the influence of a lot of possible effectors on lysosomal proteinases from rat liver. All as yet tested inhibitors of serine proteinases did not
Determination of native oligomeric state and substrate specificity of rat NTPDase1 and NTPDase2 after heterologous expression in Xenopus oocytes
Failer UB, et al.
European Journal of Biochemistry, 270(8), 1802-1809 (2003)
Simultaneous determination of ciclesonide and its active metabolite desisobutyryl-ciclesonide in human plasma by LC?APCI-MS/MS: Application to pharmacokinetic study in healthy Chinese volunteer
Su X-M, et al.
Journal of Pharmaceutical and Biomedical Analysis, 55(1), 230-235 (2011)
Markwardt, F., et al.
Thrombosis Research, 2, 343-343 (1973)
Pefabloc, 4-[2-aminoethyl] benzenesulfonyl fluoride, is a new, potent nontoxic and irreversible inhibitor of PAF-degrading acetylhydrolase
Dentan C, et al.
Biochimica et Biophysica Acta, 1299(3), 353-357 (1996)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service