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Millipore

Bile esculin azide agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Enterococcus Selective Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
beef extract, 5.0 g/L
casein enzymic hydrolysate, 17.0 g/L
esculin, 1.0 g/L
ferric ammonium citrate, 0.5 g/L
oxgall, 10.0 g/L
proteose peptone, 3.0 g/L
sodium azide, 0.15 g/L
sodium chloride, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable

application(s)

clinical testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Enterococcus spp.
selective and differential for Streptococcus spp.

Related Categories

General description

Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.

Application

Bile Esculin Azide Agar is a selective medium used for isolation and presumptive identification of faecal Streptococci.

Preparation Note

Suspend 56.65g in 1 l distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae.
Lindell, SHIRLEY S., and P. A. T. R. I. C. I. A. Quinn.
Journal of Clinical Microbiology, 1(5) , 440-443 (1975)

Articles

Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

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