Skip to Content
MilliporeSigma
All Photos(1)

Documents

85463

Millipore

Simmons Citrate Agar

NutriSelect® Basic, suitable for microbiology

Synonym(s):

Citrate Utilization Test

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

100

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
ammonium dihydrogen phosphate, 0.2 g/L
bromothymol blue, 0.08 g/L
disodium ammonium phosphate, 0.8 g/L
magnesium sulfate heptahydrate, 0.2 g/L
sodium chloride, 5 g/L
trisodium citrate, 2 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

final pH

~7.0

application(s)

agriculture
clinical testing
environmental
food and beverages
water monitoring
microbiology

suitability

nonselective and differential for Escherichia coli
nonselective and differential for Salmonella spp.
nonselective and differential for coliforms
nonselective and differential for molds (General Media)
nonselective and differential for yeasts (General Media)

Related Categories

General description

Simmons Citrate Agar uses citric acid as the carbon source. It is used to differentiate Salmonella groups from each other. It does not allow the growth of Shigella or coliforms.

Application

Simmons Citrate Agar can be used for differentiating between fecal coli and members of the aerogenes group based on citrate utilization. It can also be used for the identification of certain fungi and fungi imperfecti. The agar has been used to perform a citrate test.

Preparation Note

Suspend 23.3 g in 1 litre distilled water and heat to boiling to dissolve. Distribute in tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes. Allow tubes to cool as slants.

Other Notes

Selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

R.K. Dart
Microbiology for the Analytical Chemist (1996)
E Van Kregten et al.
Journal of clinical microbiology, 20(5), 936-941 (1984-11-01)
A culture medium was developed which selectively favored the growth of Klebsiella pneumoniae and Klebsiella oxytoca in Escherichia coli-rich fecal cultures, without the use of antibiotics. The discriminative capacity of this medium was based on the presence of only two
Elmer W. Koneman
Koneman's Color Atlas and Textbook of Diagnostic Microbiology (2006)
Massilia aurea sp. nov., isolated from drinking
water
Virginia Gallego
International Journal of Systematic and Evolutionary Microbiology (2006)

Articles

Differentiation of Escherichia coli from coliforms

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types, Salmonella enteritidis and Salmonella typhimurium, are responsible for about half of all human infections. Most outbreaks of Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on nearly any food. Chicken, eggs and their derivative products are particularly high risk.

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service