Skip to Content
MilliporeSigma
All Photos(1)

Documents

27048

Millipore

Christensen′s Urea Agar

suitable for microbiology, NutriSelect® Basic

Synonym(s):

Urea Agar Base acc. to Christensen, Urea Test Agar

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

agency

according to GB 4789.4-2016
according to ISO 10273:2017
according to ISO 19250:2010
according to ISO 6579-1:2017

Quality Level

200

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
gelatine peptone, 1 g/L
D(+)-glucose, 1 g/L
phenol red, 0.012 g/L
potassium dihydrogen phosphate, 2 g/L
sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Proteus spp.
nonselective and differential for Yersinia spp.
nonselective and differential for coliforms

Related Categories

Application

For the differentiation of urea-metabolizing microorganisms. Weak urease producers such as klebsiella, enterobacter etc. can also be differentiated. Yersinia pseudotuberculosis (urea positive) from Yersinia pestis (urea negative) acc. to Thal and Chen (1955).

Preparation Note

Dissolve 21 g in 950 ml distilled water. Sterilize by autoclaving at 121°C for 15 minutes. After cooling to 50°C, add aseptically 50ml (10 vials) sterile filtered 40% urea solution (Cat. No. 08582) and let cool as slants.

Other Notes

Screening of enteric pathogens from stool specimens

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 6

1 of 6

GranuCult® Triple sugar iron agar pkg of 500 g, for Enterobacter spp., for Salmonella spp., for Shigella spp., degradation of sugar and accompanying acid production is detected by the pH indicator phenol red

Millipore

1.03915

GranuCult® Triple sugar iron agar

Urease Activity Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK120

Urease Activity Assay Kit

Carbohydrate Consumption Broth suitable for microbiology, NutriSelect® Plus

Millipore

07410

Carbohydrate Consumption Broth

MIO Medium suitable for microbiology, NutriSelect® Plus

Millipore

M1428

MIO Medium

Lysine Decarboxylase Broth NutriSelect® Plus, suitable for microbiology

Millipore

66304

Lysine Decarboxylase Broth

TCBS Agar NutriSelect® Plus, suitable for microbiology

Millipore

86348

TCBS Agar

Two simple tests for the differentiation of plague and pseudo tuberculosis bacilli.
E THAL et al.
Journal of bacteriology, 69(1), 103-104 (1955-01-01)
W.B. Christensen
Journal of Bacteriology, 69, 103-103 (1946)
P A Villasante et al.
Journal of clinical microbiology, 25(3), 584-585 (1987-03-01)
A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards

Articles

Differentiation of Escherichia coli from coliforms

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service