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198269

Sigma-Aldrich

Erythrosin B

certified by the Biological Stain Commission, Dye content ≥85 %

Synonym(s):

Erythrosin extra bluish, 2′,4′,5′,7′-Tetraiodofluorescein disodium salt, Acid Red 51, Iodoeosin

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About This Item

Empirical Formula (Hill Notation):
C20H6I4Na2O5
CAS Number:
16423-68-0
Molecular Weight:
879.86
Colour Index Number:
45430
Beilstein/REAXYS Number:
1443945
EC Number:
240-474-8
MDL number:
MFCD00144257
UNSPSC Code:
12171500
PubChem Substance ID:
24851871
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥85%

color

dark red

pH

6.4 (30.3 °C)

mp

315 °C

solubility

H2O: 1 mg/mL, clear

density

0.625 — 0.731 g/cm3 at 30.1 °C (86.2 °F)

λmax

525 nm

ε (extinction coefficient)

≥13000 at 308-312 nm
≥32000 at 259-263 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(I)cc2c(Oc3c(I)c([O-])c(I)cc3C24OC(=O)c5ccccc45)c1I

InChI

1S/C20H8I4O5.2Na/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20;;/h1-6,25-26H;;/q;2*+1/p-2

InChI key

RAGZEDHHTPQLAI-UHFFFAOYSA-L

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Application

Erythrosin B has been used as a dye in fluorescence lifetime imaging.

Biochem/physiol Actions

Erythrosin B, also called as eosin B, is a xanthene dye. It belongs to the family of fluorescein dyes. Erythrosin B is an artificial food dye that induces hyperkinesis when swallowed by susceptible children. It plays a major role in inhibiting dopamine uptake and high affinity 3H-ouabain binding and ion transport in synaptosomes from rat caudate nucleus.

Suitability

Counterstain to alum-hematoxylin.

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

359.6 °F - closed cup

flash_point_c

182 °C - closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Phosphorescence depolarization and the measurement of rotational motion of proteins in membranes.
C Moore et al.
FEBS letters, 108(1), 161-166 (1979-12-01)
P Johnson et al.
The Biochemical journal, 203(1), 313-321 (1982-04-01)
We have previously described a method for measuring the rotational diffusion of membrane proteins by using fluorescent triplet probes [Johnson & Garland (1981) FEBS Lett. 135, 252-256]. We now describe the criteria by which the suitability of such probes may
C. R. Acad. Sci. (D), 179, 367-367 (1924)
Erythrosin B inhibits dopamine transport in rat caudate synaptosomes.
Lafferman JA and Silbergeld EK.
Science, 205(4404), 410-412 (1979)
Bright monomeric red fluorescent protein with an extended
fluorescence lifetime
Merzlyak EM
Nature Methods, 4(7), 555-557 (2007)
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