Skip to Content
MilliporeSigma
All Photos(2)

Documents

341088

Sigma-Aldrich

1,9-Dimethyl-Methylene Blue zinc chloride double salt

Dye content 80 %

Synonym(s):

DMMB, Taylor’s Blue

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Empirical Formula (Hill Notation):
C18H22ClN3S · 0.5ZnCl2
CAS Number:
931418-92-7
Molecular Weight:
416.05
MDL number:
MFCD19686959
UNSPSC Code:
12171500
PubChem Substance ID:
24860900
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 80%

impurities

<20% water

mp

250 °C (dec.) (lit.)

λmax

649 nm

ε (extinction coefficient)

≥10000 at 243-249 nm in H2O
≥18000 at 285-291 nm in H2O
≥25000 at 594-600 nm in H2O
≥35000 at 646-652 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CN(C)c1cc(C)c2N=C3C(C)=C\C(C=C3Sc2c1)=[N+](/C)C.CN(C)c4cc(C)c5N=C6C(C)=C\C(C=C6Sc5c4)=[N+](/C)C

InChI

1S/2C18H22N3S.4ClH.Zn/c2*1-11-7-13(20(3)4)9-15-17(11)19-18-12(2)8-14(21(5)6)10-16(18)22-15;;;;;/h2*7-10H,1-6H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

MKLBAKXCEQNMBC-UHFFFAOYSA-J

Related Categories

Application

1,9-Dimethyl-Methylene Blue zinc chloride double salt has been used for the quantification of sulfated glycosaminoglycans.
DMMB is a cationic dye that specifically binds to sulfated glycosaminoglycans (sGAG) with an absorbance at ~525 nm. It has been used to quantitate glycosaminoglyans such as chondroitin sulfate, keratin sulfate, and heparan sulfate and sGAG chains in proteoglycans. One limitation to this method The sGAG-DMMB complex is unstable in solution and may precipitate. Precipitation of the complex was shown to improve stability.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 4

1 of 4

Anti-NG2 Antibody, clone 1L2, ZooMAb&#174; Rabbit Monoclonal recombinant, expressed in HEK 293 cells

Sigma-Aldrich

ZRB5320

Anti-NG2 Antibody, clone 1L2, ZooMAb® Rabbit Monoclonal

Globotriaosylsphingosine from porcine blood

Sigma-Aldrich

G9534

Globotriaosylsphingosine from porcine blood

Chondroitin sulfate sodium United States Pharmacopeia (USP) Reference Standard

USP

1133570

Chondroitin sulfate sodium

Sodium formate ACS reagent, &#8805;99.0%

Sigma-Aldrich

247596

Sodium formate

Yi He et al.
FEBS open bio, 9(6), 1144-1152 (2019-05-01)
Osteoarthritis (OA) is a chronic degenerative disease that commonly affects the elderly. Current drug therapies for treating OA may cause adverse side effects, and so there remains a need to develop alternative treatments. Bergapten (BG) is a coumarin phytohormone that
P Behrendt et al.
Osteoarthritis and cartilage, 24(11), 1981-1988 (2016-10-21)
The aim of this study was to examine whether anti-inflammatory interleukin-10 (IL-10) exerts chondroprotective effects in an in vitro model of a single mechanical injury of mature articular cartilage. Articular cartilage was harvested from the femoro-patellar groove of adult cows (Bos
Makwese Maepa et al.
Tissue engineering. Part A, 25(15-16), 1137-1145 (2018-12-05)
Eucomis autumnalis is one plant that is used by various traditional healers to alleviate the signs and symptoms associated with osteoarthritis. Although the exact mechanisms remain unknown, we hypothesized that this plant can induce chondrogenesis. In this work, we explored
Anita Krouwels et al.
Spine, 43(5), 307-315 (2015-04-10)
An in vitro study using human degenerated nucleus pulposus cells. To determine the effect of osmolality and different osmolytes on the regeneration by human nucleus pulposus cells through gene expression and extracellular matrix production. Intervertebral disc (IVD) degeneration is a
Ana C Zarpelon et al.
Inflammation, 40(6), 2020-2032 (2017-08-07)
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by debilitating pain, cartilage destruction, and loss of joint function. Management of RA includes drugs that target NF-κB and downstream cytokine production. Therefore, molecules that act by inhibiting this signaling pathway
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service