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Bolton Broth, Base

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Bolton Selective Enrichement Broth, Base

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

agency

according to ISO 10272-1:2017

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

enzymatic digest of animal tissues, 10 g/L
α-ketoglutaric acid, 1.0 g/L
lactalbumin hydrolysates, 5.0 g/L
sodium carbonate, 0.6 g/L
sodium chloride, 5.0 g/L
sodium metabisulfite, 0.5 g/L
sodium pyruvate, 0.5 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
food and beverages
pathogen testing
sample preparation
microbiology

suitability

selective for Campylobacter spp.

Related Categories

General description

Bolton broth base is used for the selective enrichment of Campylobacter species from foods as recommended by ISO Committee under specifications ISO 10272-1:2006 (E).

Application

for the selective enrichment of Campylobacter species from foods

Preparation Note

Suspend 13.8 grams in 472.5 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1vial of Bolton Selective Supplement (Cat. No. 40568) and 25 ml of sterile lysed defribinated horse blood. Mix well before dispensing into sterile tubes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCF8639
BCBZ4450
BCBT7421
BCBP5374V
BCBM6819V

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Sigma-Aldrich

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Articles

On the Trail of Campylobacter

On the Trail of Campylobacter - The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis. However, other species, like C. fetus, which causes spontaneous abortions, have also been associated with human illness. Campylobacter are Gram-negative, spiral-shaped, microaerophilic and motile bacteria with uni- or bi-polar fl agella

Campylobacter Detection: Rapid Testing Solutions for the Detection of Campylobacter

Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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