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861111

Sigma-Aldrich

Bismarck Brown Y

certified by the Biological Stain Commission, Dye content 50 %

Synonym(s):

4,4′-(m-Phenylenebisazo)bis-m-phenylenediamine dihydrochloride, Basic Brown 1, Bismarck Brown B, Vesuvine

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About This Item

Empirical Formula (Hill Notation):
C18H18N8 · 2HCl
CAS Number:
10114-58-6
Molecular Weight:
419.31
Colour Index Number:
21000
EC Number:
233-314-3
MDL number:
MFCD00012977
UNSPSC Code:
12171500
PubChem Substance ID:
24888530
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

100

form

powder

composition

Dye content, 50%

solubility

H2O: 10 mg/mL, clear to turbid, red-orange to red

λmax

457 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl.Cl.Nc1ccc(\N=N\c2cccc(c2)\N=N\c3ccc(N)cc3N)c(N)c1

InChI

1S/C18H18N8.2ClH/c19-11-4-6-17(15(21)8-11)25-23-13-2-1-3-14(10-13)24-26-18-7-5-12(20)9-16(18)22;;/h1-10H,19-22H2;2*1H/b25-23+,26-24+;;

InChI key

MCZVRBLCRZWFJH-SPBSJSFYSA-N

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Related Categories

Application

Bismarck Brown Y is used for the staining of plant samples, including stem, root, starch granules and epidermis.

Suitability

Certified for use in staining goblet cells and cartilage.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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R.D. Lillie Ed.
Conn?s Biological Stains, 145-145 (1977)
The use of Bismarck brown Y in some new stain schedules.
Blaydes GW
Stain Technology, 14.3, 105-110 (1939)
The Journal of Laboratory and Clinical Medicine, 26, 1200-1200 (1941)
[Case of poisoning by the dye Bismark Brown].
N M Ageeva et al.
Sudebno-meditsinskaia ekspertiza, 29(3), 59-59 (1986-07-01)
P Talbot et al.
Stain technology, 56(5), 307-309 (1981-09-01)
We have previously described a triple stain for evaluating normal acrosome reactions of human sperm. This procedure uses trypan blue to distinguish live and dead sperm, Bismarck brown to stain the sperm's postacrosomal region, and rose Bengal to stain the
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