Skip to Content
MilliporeSigma
All Photos(2)

Documents

A8456

Sigma-Aldrich

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride

≥97.0% (HPLC)

Synonym(s):

AEBSF

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Empirical Formula (Hill Notation):
C8H10FNO2S · HCl
CAS Number:
30827-99-7
Molecular Weight:
239.69
Beilstein/REAXYS Number:
7604627
MDL number:
MFCD00132962
UNSPSC Code:
12352202
PubChem Substance ID:
24278240
NACRES:
NA.77

biological source

synthetic (organic)

Quality Level

300

assay

≥97.0% (HPLC)

form

powder

mp

183-191  °C

solubility

H2O: 50 mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)

storage temp.

−20°C

SMILES string

FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl

InChI

1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H

InChI key

WRDABNWSWOHGMS-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used:
  • as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation
  • as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity
  • as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates

Recommended concentrations for use are 0.1-1 mM.

Biochem/physiol Actions

AEBSF can inhibit acetylcholinesterase (AChE).
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 7

1 of 7

Pepstatin A microbial, ≥90% (HPLC)

Sigma-Aldrich

P5318

Pepstatin A

Bestatin Hydrochloride InSolution, ≥98%, a metalloprotease inhibitor

Sigma-Aldrich

200488

Bestatin Hydrochloride

3,4-Dichloroisocoumarin serine protease inhibitor

Sigma-Aldrich

D7910

3,4-Dichloroisocoumarin

PMSF Phenylmethylsulfonyl fluoride

Roche

PMSF-RO

PMSF

Aprotinin from bovine lung lyophilized powder, 3-8 TIU/mg solid

Sigma-Aldrich

A1153

Aprotinin from bovine lung

Antipain dihydrochloride from microbial source protease inhibitor

Sigma-Aldrich

A6191

Antipain dihydrochloride from microbial source

Phenylmethanesulfonyl fluoride ≥98.5% (GC)

Sigma-Aldrich

P7626

Phenylmethanesulfonyl fluoride

Ilario Mennella et al.
The Journal of nutrition, 145(9), 2169-2175 (2015-07-17)
Food palatability increases food intake and may lead to overeating. The mechanisms behind this observation are still largely unknown. The aims of this study were the following: 1) to elucidate the plasma responses of endocannabinoids, N-acylethanolamines, and gastrointestinal peptides to
Ignacio Pedrós et al.
Biochimica et biophysica acta, 1842(9), 1556-1566 (2014-06-03)
The present study had focused on the behavioral phenotype and gene expression profile of molecules related to insulin receptor signaling in the hippocampus of 3 and 6 month-old APPswe/PS1dE9 (APP/PS1) transgenic mouse model of Alzheimer's disease (AD). Elevated levels of
Sara Lind Jepsen et al.
The Journal of clinical endocrinology and metabolism, 105(1) (2019-10-15)
The gastrointestinal hormone ghrelin stimulates growth hormone secretion and appetite, but recent studies indicate that ghrelin also stimulates the secretion of the appetite-inhibiting and insulinotropic hormone glucagon-like peptide-1 (GLP-1). To investigate the putative effect of ghrelin on GLP-1 secretion in
Niina Aaltonen et al.
Biological procedures online, 22, 6-6 (2020-03-20)
Serine hydrolases (SHs) are a functionally diverse family of enzymes playing pivotal roles in health and disease and have emerged as important therapeutic targets in many clinical conditions. Activity-based protein profiling (ABPP) using fluorophosphonate (FP) probes has been a powerful
Robert J Snelgrove et al.
The Journal of allergy and clinical immunology, 134(3), 583-592 (2014-03-19)
The fungal allergen Alternaria alternata is implicated in severe asthma and rapid onset life-threatening exacerbations of disease. However, the mechanisms that underlie this severe pathogenicity remain unclear. We sought to investigate the mechanism whereby Alternaria was capable of initiating severe
View All Related Papers

Articles

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Related Content

Trypsin Serine Protease Enzyme

Trypsin is an enzyme in the serine protease class that consists of a polypeptide chain of 223 amino acid residues. Multiple sources, grades and formulations of trypsin specifically designed for research applications are available.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service