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B9431

Sigma-Aldrich

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

Synonym(s):

5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate

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About This Item

Empirical Formula (Hill Notation):
C33H32N4O8S
Molecular Weight:
644.69
UNSPSC Code:
12171500
eCl@ss:
34058011
PubChem Substance ID:
329773319
NACRES:
NA.47

product name

Biotin-4-Fluorescein, ≥95.0% (HPLC)

Quality Level

100

assay

≥95.0% (HPLC)

form

powder

mol wt

644.71

color

light orange to very dark orange
light yellow to very dark yellow

ε (extinction coefficient)

≥70 at 491-497 nm in phosphate buffer at 0.05 M

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56

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Application

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.
Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

Biochem/physiol Actions

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Sivaraj Sivaramakrishnan et al.
The Journal of cell biology, 187(1), 53-60 (2009-09-30)
Unconventional myosins interact with the dense cortical actin network during processes such as membrane trafficking, cell migration, and mechanotransduction. Our understanding of unconventional myosin function is derived largely from assays that examine the interaction of a single myosin with a
Cell-Adhesion Patterning by using Carbon Negative-Ion Implantation into Albumin and Avidin Layers on Polystyrene.
Sommani P, et al.
Transactions of the Materials Research Society of Japan, 36.3, 289-292 (2011)
Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization.
Kada G, et al.
Biochimica et Biophysica Acta, 1427, 44-48 (1999)
Nathaniel G Hentz et al.
Journal of laboratory automation, 19(2), 153-162 (2013-09-14)
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays

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