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104-05A

Sigma-Aldrich

Human Epidermal Melanocytes: HEM, adult

Synonym(s):

HEM cells

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human skin (normal abdominal)

Quality Level

100

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

Epidermal

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metabolic disorders

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Epidermal melanocytes are pigment-producing cells located at the basal level of epidermis, where they interact with keratinocytes via cellular processes called dendrites. Melanin, the pigment produced by melanocytes and responsible for skin color, is then transferred to keratinocytes, where it is stored in vesicles called melanosomes located around the nucleus to provide protection from UV radiation. HEM maintain their characteristic shape in culture for many generations. They produce melanin and serve as a useful cell model for the studies of melanocyte proliferation and differentiation, as well as progression of melanocytic neoplasia.

Cell Line Origin

Skin

Application

melanin production, melanocyte proliferation, differentiation

Components

Basal Medium containing 30% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO
  • Can be cultured at least 12 doublings

Subculture Routine

Please refer to the HEM Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Protocols

Human Epidermal Melanocytes (HEM) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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