Skip to Content
MilliporeSigma
All Photos(1)

Documents

19123

Sigma-Aldrich

Nile Red

suitable for fluorescence, BioReagent, ≥97.0% (HPLC)

Synonym(s):

Nile Blue A Oxazone

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
7385-67-3
Molecular Weight:
318.37
Beilstein/REAXYS Number:
279110
EC Number:
230-966-0
MDL number:
MFCD00011639
UNSPSC Code:
12161900
PubChem Substance ID:
57647780
NACRES:
NA.25

product line

BioReagent

Quality Level

100

assay

≥97.0% (HPLC)

form

solid

mp

203-205 °C (lit.)
205-215 °C

λmax

553 nm

fluorescence

λex 530 nm; λem 635 nm in methanol

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Nile Red is an uncharged hydrophobic molecule and a benzophenoxazone dye. It functions as a fluorescent probe for intracellular lipids and hydrophobic domains of proteins. This dye is fluorescent in all organic solvents. Its fluorescence colors range from golden yellow to deep red.

Application

Nile Red has been used for the staining of intracellular lipid droplets and neutral lipids.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 3

1 of 3

Oil Red O certified by the Biological Stain Commission

Sigma-Aldrich

O0625

Oil Red O

Fluorescein 5(6)-isothiocyanate BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

Sigma-Aldrich

46950

Fluorescein 5(6)-isothiocyanate

Rhodamine B for fluorescence

Sigma-Aldrich

83689

Rhodamine B

Biosynthesis of high yield fatty acids from Chlorella vulgaris NIES-227 under nitrogen starvation stress during heterotrophic cultivation
Shen XF, et al.
Water Research, 81(3), 294-300 (2015)
Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces
Sackett D L and Wolff J
Analytical Biochemistry, 167(2), 228-234 (1987)
Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
Feaver RE, et al.
JCI insight, 1(20) (2016)
Resveratrol suppresses T0901317-induced hepatic fat accumulation in mice
Gao M and Liu D
The AAPS Journal, 15, 744-752 (2013)
Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes
Kim K, et al.
Scientific Reports, 6, 36815-36815 (2016)
View All Related Papers

Articles

Stimuli-Responsive Materials as Intelligent Drug Delivery Systems

By altering the physicochemical properties, smart or intelligent drug delivery systems can be designed to deliver therapeutic molecules on-demand. Learn more about the application of stimuli-responsive materials in drug delivery.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service