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N5632

Sigma-Aldrich

Nile Blue A

Dye content ≥75 %, Powder

Synonym(s):

Basic Blue 12, Nile blue sulfate

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About This Item

Linear Formula:
2C20H20N3O · SO4
CAS Number:
3625-57-8
Molecular Weight:
732.85
Colour Index Number:
51180
EC Number:
222-832-5
MDL number:
MFCD00064529
UNSPSC Code:
12171500
PubChem Substance ID:
24897743
NACRES:
NA.47

product name

Nile Blue A, Dye content ≥75 %

form

powder

Quality Level

100

composition

Dye content, ≥75%

mp

>300 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL, blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78

InChI

1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)

InChI key

QIRDPEPUXNCOLD-UHFFFAOYSA-N

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General description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Application

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Features and Benefits

Used in the preparation of an amperometric glucose sensor.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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