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Sigma-Aldrich

Nile Blue chloride

Dye content 85 %

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About This Item

Empirical Formula (Hill Notation):
C20H20ClN3O
CAS Number:
2381-85-3
Molecular Weight:
353.85
Colour Index Number:
51180
Beilstein/REAXYS Number:
3923952
MDL number:
MFCD00011936
UNSPSC Code:
12171500
PubChem Substance ID:
24853342
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 85%

solubility

water: 1 mg/mL, opaque, blue to very deep blue

λmax

638 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34

InChI

1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H

InChI key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

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Application

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

Biochem/physiol Actions

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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