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50981

Sigma-Aldrich

Guanidine thiocyanate

BioReagent, for molecular biology, ≥99%

Synonym(s):

Guanidinium rhodanide, Guanidinium thiocyanate

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About This Item

Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
593-84-0
Molecular Weight:
118.16
Beilstein/REAXYS Number:
3563461
EC Number:
209-812-1
MDL number:
MFCD00013027
UNSPSC Code:
12352107
PubChem Substance ID:
57651291
NACRES:
NA.52

biological source

synthetic

Quality Level

200

grade

for molecular biology

product line

BioReagent

assay

≥99%

form

powder

technique(s)

RNA extraction: suitable

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

color

colorless to white,

mp

115-122 °C
120-122 °C (lit.)

solubility

H2O: 4 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤5000 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

storage temp.

room temp

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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Application

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.
Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.

Principle

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

EUH032

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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