G1N350
GenElute™ Mammalian Genomic DNA Miniprep Kits
sufficient for 350 purifications
Synonym(s):
Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute
About This Item
Recommended Products
usage
sufficient for 350 purifications
Quality Level
technique(s)
DNA purification: suitable
storage temp.
15-25°C
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General description
Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Application
- to extract DNA from fin samples
- to extract the BSTUI restriction enzyme from the DNA
- to isolate DNA from the blood samples extracted from caudal fin
- to extract tissue and hair follicle DNA
- restriction endonuclease digestions
- PCR
- Southern blots
- sequencing reactions
- cloning
- ligation
Features and Benefits
- Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
- Elution volume: 200 - 400 μl
- Time required: 20 min after lysis
- A260/A280 ratio: 1.6 - 1.9
- Mechanical homogenization required: No
Principle
Other Notes
Legal Information
recommended
related product
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible, corrosive hazardous materials
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Protocols
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
The GenElute Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
Related Content
The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian cells and tissues. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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