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P9172

Sigma-Aldrich

Pyronin Y

for NA electrophoresis

Synonym(s):

Pyronin Y, Pyronin G, Pyronin J

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About This Item

Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
Colour Index Number:
45005
Beilstein/REAXYS Number:
3795789
EC Number:
202-147-8
MDL number:
MFCD00011725
UNSPSC Code:
12352200
PubChem Substance ID:
57654569
NACRES:
NA.52

grade

for molecular biology

Quality Level

200

form

powder

composition

Dye content, ≥45%

technique(s)

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

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Application

Pyronin Y has been used:
  • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)
  • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)
  • staining of CD34+ cells

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Green, F.J., ed.
The Sigma-Aldrich Handbook of Stains, Dyes and Indicators,, 601-602 (1990)
A gel electrophoresis method for determining the relative binding constants of biologically active, intercalating fluorescent stains.
S M Hassur et al.
Analytical biochemistry, 95(2), 329-339 (1979-06-01)
Senescence and apoptosis block hematopoietic activation of quiescent hematopoietic stem cells with short telomeres
Wang J, et al.
Blood, 124(22), 3237-3240 (2014)
Photosensitizing effects of the tricyclic heteroaromatic cationic dyes pyronin Y and toluidine blue O (tolonium chloride
Darzynkiewicz Z and Carter SP
Cancer research, 48(5) (1988)
Staining of RNA after polyacrylamide gel electrophoresis.
K Marcinka
Methods in cell biology, 10, 49-68 (1975-01-01)
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