A4414
Albumin from porcine serum
lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
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About This Item
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biological source
Porcine
Quality Level
assay
≥99% (agarose gel electrophoresis)
form
lyophilized powder
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
Porcine ... ALB(396960)
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General description
Albumin is present abundantly in serum. Porcine serum albumin (PSA) comprises a flexible C-terminal region compared to other albumins.
Application
Albumin from porcine serum has been used:
- as a non-hazardous protein to test its effect on T84 polarized intestinal monolayers and Caco2 polarized intestinal monolayers barrier integrity in trans-Epithelial Electrical Resistance (TEER) assay
- to test its effect on the human intestinal epithelial cell lines
- to test its interaction with the biodegradable ester-functionalized gemini surfactant 16-E2-16 using ultraviolet (UV)-visible and circular dichroism (CD) spectroscopy measurements
Biochem/physiol Actions
Albumin binds to many blood associated proteins. Porcine serum albumin (PSA) is regarded as a major allergen. PSA is used in the immunosensors, molecularly imprinted nanogels (MIP-NGs) and enzyme-linked immunosorbent assay (ELISA) protein-based detection for assessing contamination, especially in meat products.
Preparation Note
Prepared from albumin (A2764).
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Korean journal for food science of animal resources, 34(3), 355-361 (2014-01-01)
This study was conducted to evaluate the changes in porcine serum albumin (PSA), a major allergen, which occur when raw pork ham is marinated with kiwi or pineapple juice, and/or when the ham is pan broiled at 300℃ for 4
Analytical chemistry, 92(9), 6401-6407 (2020-04-14)
Accurate, simple, and valuable analytical methods for detection of food contamination are rapidly expanding to evaluate the validity of food product quality because of ethnic considerations and food safety. Herein molecularly imprinted nanogels (MIP-NGs), capable of porcine serum albumin (PSA)
Nature protocols, 7(2), 340-350 (2012-01-28)
We describe a method for the efficient and selective identification of DNA containing the 5-hydroxymethylcytosine (5-hmC) modification. This protocol takes advantage of two proteins: T4 β-glucosyltransferase (β-gt), which converts 5-hmC to β-glucosyl-5-hmC (β-glu-5-hmC), and J-binding protein 1 (JBP1), which specifically
Free radical biology & medicine, 47(5), 600-607 (2009-06-09)
Although the glycocalyx has been implicated in wall shear stress (WSS) mechanotransduction, the role of glycocalyx components in nitric oxide (NO) and reactive oxygen species (ROS) production remains unclear. Here, we tested the hypothesis that glycocalyx is implicated in both
Neuroscience, 152(1), 70-81 (2008-01-29)
The notion of functional interactions between the alpha7 nicotinic acetylcholine (alpha7 nACh) and the cannabinoid systems is emerging from recent in vitro and in vivo studies. Both the alpha7 nACh receptor and the cannabinoid receptor 1 (CB1) are highly expressed
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