A5102
Anti-Aurora B antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-AIM-1, Anti-AIR-2 Kinase, Anti-AIRK-2
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 41 kDa
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
technique(s)
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:50 using HeLa cells
microarray: suitable
western blot: 1:1,000 using nuclei-enriched fraction of mouse NIH-3T3 cells
western blot: 1:1,000 using using PC-12 rat phaeochromocytoma cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AURKB(9212)
mouse ... Aurkb(20877)
rat ... Aurkb(114592)
Related Categories
General description
Aurora B (AIRK2, AIR-2 kinase, AIM-1) is a serine/threonine kinase. Aurora B is evolutionally conserved from yeast to human. The Drosophila serine/threonine protein kinase Aurora and the S. cerevisiae IpI1 kinase are highly homologous to human Aurora B.
Specificity
Anti-Aurora B recognizes human, mouse, and rat Aurora B/AIR-2 Kinase enzymes. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.
Immunogen
synthetic peptide corresponding to amino acid residues 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH.
Application
Anti-Aurora B antibody has been used in:
- in immunoblotting
- in immunoprecipitation
- in immunofluorescence
- in immunofluorescence staining
- in western blotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Rabbit polyclonal anti-Aurora B antibody is used to tag Aurora B/AIR-2 Kinase for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Aurora B/AIR-2 Kinase in chromosome segregation, cytokinesis, and cancer development.
Biochem/physiol Actions
Aurora B plays key roles in chromosome segregation, cytokinesis, and cancer development. It also plays a role in chromosomal condensation by phosphorylating the H3 histone. In C. elegans, Aurora-B is required for normal localization and function of the ZEN-4/CeMKLP (Kinesin like protein/mitotic kinesin-like protein), a kinesin-related protein essential for completion of cytokinesis. Loss of the Aurora B kinase results in chromosome segregation defects and failures in cytokinesis. Aurora B displays a localization pattern typical of chromosomal passenger proteins as the inner centromeric protein (INCENP), TD-60 and Survivin. INCENP and Survivin interact directly with Aurora B. Chromosomal passenger proteins undergo dynamic redistribution during mitosis. They localize at centromers during prometaphase and relocate to midzone microtubules and midbodies during anaphase and telophase. The mRNA and protein levels of Aurora B are induced during G2M and decrease rapidly after the end of mitosis. Levels of Aurora B are increased in several human cancer cell lines.
Target description
Auroa B is a member of a family of Auora kinases that function for proper chromosomal segregation during mitosis. Auora B localizes specifically to the kinetochores of microtubules.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Yu-Chu Wang et al.
Experimental & molecular medicine, 50(6), 70-70 (2018-06-10)
Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis
Validating Aurora B as an anti-cancer drug target
Girdler F, et al.
Journal of Cell Science, 119(17), 3664-3675 (2006)
Shi-Zheng Wu et al.
Cell biochemistry and function, 35(2), 113-123 (2017-02-25)
It has been reported that CXCR4-overexpressing mesenchymal stem cells (MSCCX4 ) can repair heart tissue post myocardial infarction. This study aims to investigate the MSCCX4-derived paracrine cardio-protective signaling in the presence of myocardial infarction. Mesenchymal stem cells (MSCs) were divided
Aurora B regulates MCAK at the mitotic centromere
Andrews P D, et al.
Developmental Cell, 6(2), 253-268 (2004)
RNAi-mediated knockdown of AURKB and EGFR shows enhanced therapeutic efficacy in prostate tumor regression
Addepalli M K, et al.
Gene Therapy, 17(3), 352-352 (2010)
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