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A6270

Sigma-Aldrich

Azure A chloride

Dye content ≥70 %, Powder or crystals

Synonym(s):

Azure eosin methylene blue; 7-Amino-N,N-dimethyl-3H-phenothiazin-3-iminium chloride

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About This Item

Empirical Formula (Hill Notation):
C14H14ClN3S
CAS Number:
531-53-3
Molecular Weight:
291.80
Colour Index Number:
52005
Beilstein/REAXYS Number:
3922287
EC Number:
208-510-7
MDL number:
MFCD00012112
UNSPSC Code:
12171500
PubChem Substance ID:
24891073
NACRES:
NA.47

product name

Azure A chloride, Dye content ≥70 %

form

powder or crystals

Quality Level

200

composition

Dye content, ≥70%

color

green to very dark green

mp

290 °C (dec.) (lit.)

solubility

water: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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Application

Azure A chloride has been used for the staining of polyacrylamide gel to analyse the bovine lung heparin digestion by heparinase I. It has been used for the determination of the sulphated groups content in polysaccharides.

Biochem/physiol Actions

Azure A is a basic cationic dye. The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.

Caution

Handle and store under argon. Glove bag item.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Generating heparan sulfate saccharide libraries for glycomics applications.
Powell AK, et al.
Nature Protocols, 5, 821-833 (2010)
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