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A4043

Sigma-Aldrich

Azure B

Dye content, ≥89%, certified by the Biological Stain Commission, powder

Synonym(s):

N,N,N′-Trimethylthionin, Azure I, Methyleneazure

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
531-55-5
Molecular Weight:
305.83
Colour Index Number:
52010
Beilstein/REAXYS Number:
3922630
EC Number:
208-511-2
MDL number:
MFCD00011935
UNSPSC Code:
12171500
PubChem Substance ID:
24890740
NACRES:
NA.47

product name

Azure B, certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥89%

color

dark green

mp

205-210 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

fluorescence

λex 648 in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

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Application

Azure B has been used for the staining of chromosomes.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

Suitability

Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nuclear DNA content, base composition, and cytogenetic characterization of Christia obcordata.
Sakhanokho HG and Islam-Faridi N
J. Am. Soc. Hort. Sci., 138, 205-209 (2013)
Bain BJ, et al.
Dacie and Lewis Practical Haematology (2016)
Cell and Tissue Culture in Forestry (2012)
Anél Petzer et al.
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Methylene blue (MB) has been shown to act at multiple cellular and molecular targets and as a result possesses diverse medical applications. Among these is a high potency reversible inhibition of monoamine oxidase A (MAO-A) that may, at least in
S Rodríguez Couto et al.
Chemosphere, 46(1), 83-86 (2002-01-25)
This work reports a preliminary design of a new photochemical reactor and its application to photochemical degradation of two dyes, Crystal Violet and Azure B, operating in both batch and continuous processes. A novel kind of photocatalyst, consisting of ZnO
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