A7064
Anti-Biotin−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Anti-Biotin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
goat
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
technique(s)
direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.
Goat Anti-Biotin-Alkaline Phosphatase antibody is specific for biotin when tested against biotin-goat IgG, goat IgG, and KLH.
Goat Anti-Biotin-Alkaline Phosphatase antibody is specific for biotin when tested against biotin-goat IgG, goat IgG, and KLH.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.
Immunogen
Biotin-KLH conjugate
Application
ELISA Assays using human spleen cells were performed using alkaline phosphatase-conjugated goat anti-biotin as the secondary antibody with incubation for 1.5 hours at room temperature. Purified IgG from K/BxN, BxSB or C57BL/6 mouse serum was analyzed by western blot using alkaline phosphatase-conjugated goat anti-biotin antibody as the secondary.
Goat Anti-Biotin-Alkaline Phosphatase antibody has been used for dot blot assays. The product can also be used for ELISA (1:30,000) and immunohistochemistry (1:50).
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificates of Analysis (COA)
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Andrea T Descheneau et al.
Genetics, 169(1), 123-135 (2004-10-07)
When the cytochrome-mediated mitochondrial electron transport chain of Neurospora crassa is disrupted, an alternative oxidase encoded by the nuclear aod-1 gene is induced. The alternative oxidase donates electrons directly to oxygen from the ubiquininol pool and is insensitive to chemicals
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Peter A Suci et al.
Chemistry & biology, 14(4), 387-398 (2007-04-28)
Nanomedicine directed at diagnosis and treatment of infections can benefit from innovations that have substantially increased the variety of available multifunctional nanoplatforms. Here, we targeted a spherical, icosahedral viral nanoplatform to a pathogenic, biofilm-forming bacterium, Staphylococcus aureus. Density of binding
Heike Albert et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(39), 15005-15009 (2008-09-26)
IgG antibodies are potent inducers of proinflammatory responses. During autoimmune diseases such as arthritis and systemic lupus erythematosus, IgG autoantibodies are responsible for the chronic inflammation and destruction of healthy tissues by cross-linking Fc receptors on innate immune effector cells.
Rocio Rodriguez-Melendez et al.
The Journal of nutritional biochemistry, 14(12), 680-690 (2003-12-24)
In mammals, biotin serves as coenzyme for four carboxylases, which play essential roles in the metabolism of glucose, amino acids, and fatty acids. Biotin deficiency causes decreased rates of cell proliferation, impaired immune function, and abnormal fetal development. Evidence is
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