SAF-1

Gilt head seabream caudal fin

SAF-1 has been developed from the fin tissues of an adult gilt-head seabream (sparius aurata) without immortalising treatments. SAF-1 supports the replication of several common fish viruses such as lymphocystis disease virus (LDV), infectious haematopoietic necrosis virus (IHNV) and viral haemorrhagic septicaemia virus (VHSV). SAF-1 has a higher susceptibility to rhabdoviruses, especially UHNV. In the SAF-1 line, neither chromosome changes or differences from passage 50-70 have been detected, suggesting a normal karyotype and stable cell line. The cell line may be useful in isolating and studying undetected latent and potential lytic viruses infecting gilt-head seabream and other related sparid species. In addition to its proven applications, SAF-1 could be used as an in vitro system to address multiple questions related to the genetic engineering of this species, such as evaluating the efficiency of the promotors in transgenic constructions, testing whether this species is susceptible to viral vectors available in fish or whether heterologous transposable elements can be mobilised for insertional mutagenesis. This cell line was mycoplasma eradicated at ECACC.

Genetic engineering, virus susceptibility, fish physiology.

L15 (L5520) + 4mM L-Glutamine (G7513) + 15% FBS / FCS (F2442)

Split sub-confluent cultures (70-80%) 1:3 -1.6 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin/EDTA; 25°C; no CO₂.

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