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product name
NBT-II, 93012901
biological source
rat bladder
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Rat Wistar bladder tumour
Cell Line Description
Derived from a male Wistar rat bladder tumour induced with N-butyl-N-(4-hydroxybutyl) nitrosamine. The histotypic differentiation normally displayed by these cells is inhibited by vitamin A and gives rise to the appearance of giant cells.
Application
Maturation of epidermoid carcinoma
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2.5x1,000,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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