TO human cell line
IHW Number: 9218, 94022549
Synonym(s):
TO-PP
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About This Item
UNSPSC Code:
41106514
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biological source
human blood
ethnicity
Not specified
gender
not specified
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
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General description
Consanguineous: No
Homozygous: No
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 6802 3001
Cw: 0602 15
B: 1302 5101
Cw: 0602 15
B: 1302 5101
Serological Profile
HLA A: 68 30
HLA A ief: 68.2
HLA C: 6
HLA B: 13 51
Bw4/bw6: 4
HLA A ief: 68.2
HLA C: 6
HLA B: 13 51
Bw4/bw6: 4
Culture Medium
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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