B16 melanoma 4A5 Cell Line from mouse
94042254, Mouse melanoma, producing melanin
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About This Item
UNSPSC Code:
41106514
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biological source
mouse skin
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Melanin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse melanoma, melanin producing
Cell Line Description
Derived from a melanoma from the skin of a C57BL/6 strain mouse, showing fibroblast-like characteristics, which produces melanin. Cells may lose ability to produce pigmentation in long term culture.
Application
B16 melanoma 4A5 cell line has been used to study the spectral and biological characteristics of copper (II) mesoporphyrinic complexes. It has also been used to evaluate the cytotoxicity of JaZ-30, a matrix metalloproteinase-2 (MMP-2) inhibitor.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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