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F2043

Sigma-Aldrich

Anti-β-Tubulin−FITC antibody, Mouse monoclonal

clone TUB 2.1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-β-Tubulin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:25 using cultured BHK cells

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB(203068) , TUBB1(81027) , TUBB1(81027) , TUBB1(81027) , TUBB1(81027) , TUBB2A(7280) , TUBB2A(7280) , TUBB2A(7280) , TUBB2A(7280) , TUBB2C(10383) , TUBB2C(10383) , TUBB2C(10383) , TUBB2C(10383)
mouse ... Tubb1(104068) , Tubb1(104068) , Tubb1(104068) , Tubb1(104068) , Tubb2a(22151) , Tubb2a(22151) , Tubb2a(22151) , Tubb2a(22151) , Tubb2c(227613) , Tubb2c(227613) , Tubb2c(227613) , Tubb2c(227613) , Tubb3(22152)
rat ... Tubb2(29212) , Tubb2(29212) , Tubb2(29212) , Tubb2(29212) , Tubb2c(296554) , Tubb2c(296554) , Tubb2c(296554) , Tubb2c(296554)

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General description

β-Tubulin belongs to the subfamily of tubulins. It has two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain, which has the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). TUBB1 (tubulin beta class I) is located on human chromosome 6p21.33.(10)
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

Specificity

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Immunogen

tubulin from rat brain.

Application

FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays.
Monoclonal Anti-β-Tubulin-FITC antibody has been used:
  • in the evaluation of the status of the meiotic spindle and the arrangement of chromosomes
  • in immunofluorescence studies
  • in immunofluorescence microscopy
  • in spindle and chromosome imagery(6)

Biochem/physiol Actions

Mutation in β-Tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Transient increase in the transcript levels of gamma-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls
Soga K, et al.
Journal of Plant Research, 121(5), 493-498 (2008)
Abraham L Kierszenbaum
Molecular reproduction and development, 62(1), 1-3 (2002-04-05)
Two microtubule-containing structures are assembled during spermiogenesis: a transient manchette and a stable axoneme. Both structures contain microtubules enriched in posttranslationally modified tubulins. Despite the existence of a spectrum of tubulin isotypes postulated by the multi-tubulin hypothesis, further extended by
Peloruside-and laulimalide-resistant human ovarian carcinoma cells have betaI-tubulin mutations and altered expression of betaII-and betaIII-tubulin isotypes
Kanakkanthara A, et al.
Molecular Cancer Therapeutics, 393(6681), molcanther-molcan1057 (2011)
Changes to the meiotic spindle and zona pellucida of mature mouse oocytes following different cryopreservation methods
Ko C S, et al.
Animal Reproduction Science, 105(3-4), 272-282 (2008)
J C Bruusgaard et al.
Journal of applied physiology (Bethesda, Md. : 1985), 100(6), 2024-2030 (2006-02-25)
We have recently published a new technique for visualizing nuclei in living muscle fibers of intact animals, based on microinjection of labeled DNA into single myofibers, excluding satellite cells (Bruusgaard JC, Liestol K, Ekmark M, Kollstad K, and Gundersen K.
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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