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F7512

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)−FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Sheep Anti-Rabbit IgG (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL number:
MFCD00162786
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

sheep

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:160
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Sheep polyclonal anti-Rabbit IgG (whole molecule)−FITC antibody binds normal rabbit serum and rabbit IgG. It does not cross react with human IgG.

Application

Anti-Rabbit IgG (whole molecule)-FITC antibody produced in sheep has been used in:
  • immunocytochemistry
  • immunofluorescence
  • immunohistochemistry

Sheep polyclonal anti-Rabbit IgG (whole molecule)−FITC antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids by fluorescent techniques.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

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