F8424
Fibroblast Growth Factor-4 human
FGF-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
hFGF-4, FGF-4, K-FGF
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About This Item
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biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE and HPLC)
form
lyophilized powder
potency
≤10.0 ng/mL
quality
endotoxin tested
mol wt
19 kDa
packaging
pkg of 25 and 100 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... FGF4(2249) , FGF4(2249)
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General description
Fibroblast Growth Factor-4 (FGF4) is a 19 kDa protein, belonging to the FGF family of proteins. Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34 kDa and they possess a conserved sequence of 120 amino acids. The FGF4 gene is mapped to human chromosome 11q13.3.
Application
Fibroblast Growth Factor-4 human has been used:
- To maintain trophectoderm stem cells.
- In neurogenic differentiation.
- In hepatocyte differentiation.
Biochem/physiol Actions
Fibroblast growth factor-4 (FGF-4) is a 19 kDa protein identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development.
Fibroblast growth factor-4 (FGF-4) is identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development. FGF-4 participates in cell differentiation and cell migration.
Physical form
Lyophilized from a 0.2 μm filtered buffer solution containing a carrier protein.
Analysis Note
The biological activity is measured by the dose-dependent stimulation of thymidine uptake using BaF3 cells expressing FGF receptors.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Derivation, maintenance, and characterization of rat embryonic stem cells in vitro
Embryonic Stem Cell Protocols, 45-58 (2006)
Endometrial regenerative cells: a novel stem cell population
Meng X, et al.
Journal of Translational Medicine, 5(1), 57-57 (2007)
The oncoprotein HBXIP up-regulates FGF4 through activating transcriptional factor Sp1 to promote the migration of breast cancer cells
Shi H, et al.
Biochemical and biophysical research communications, 471(1), 89-94 (2016)
Increased FGF3 and FGF4 gene dosage is a risk factor for craniosynostosis.
Grillo L, et al.
Gene, 534(2), 435-439 (2014)
Riley McMahon et al.
Frontiers in cell and developmental biology, 9, 777652-777652 (2022-02-19)
The specification of anterior head tissue in the late gastrulation mouse embryo relies on signaling cues from the visceral endoderm and anterior mesendoderm (AME). Genetic loss-of-function studies have pinpointed a critical requirement of LIM homeobox 1 (LHX1) transcription factor in
Articles
Fibroblast growth factors in cell culture and various growth factors for your research
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