Skip to Content
MilliporeSigma
All Photos(1)

Documents

FG0465

SAFC

IMDM

With stable-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture

Pharma Manufacturing

Synonym(s):

Cell culture medium, Iscove′s MDM, Iscove’s Modified Dulbecco’s Medium, Modified Dulbecco′s medium

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

product name

Iscove′s Modified Dulbecco′s Medium, With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture

Quality Level

400

sterility

sterile-filtered
sterile

product line

AQmedia™

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

stable glutamine: yes
NaHCO3: yes
glucose: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service