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G6784

Sigma-Aldrich

L-Glutamine–Penicillin–Streptomycin solution

L-glutamine 200 mM, streptomycin 10 mg/mL, penicillin 10,000 units, 0.1 μm filtered, BioReagent, suitable for cell culture

Synonym(s):

Pen/Strep, Penicillin/Streptomycin 

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About This Item

MDL number:
MFCD00283636
UNSPSC Code:
12352207
NACRES:
NA.76

biological source

synthetic

Quality Level

300

description

stabilized

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

composition

L-glutamine, 200 mM
penicillin, 10,000 units
streptomycin, 10 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

mode of action

cell wall synthesis | interferes
protein synthesis | interferes

storage temp.

−20°C

Related Categories

Application

This antibiotic solution is used to reduce or eliminate bacterial contamination in primary cell cultures. It is recommended for us at 10 mL/L.

Biochem/physiol Actions

Mode of Action: The product acts in three ways: Glutamine is an essential energy source for most mammalian cells in culture due to its amino acid carbon skeleton. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria.

Components

This solution is formulated to contain 200 mM L-glutamine, 10,000 units of Penicillin and 10 mg/mL of streptomycin, solubilized in a proprietary buffer.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Miriam Gonzalez Gonzalez et al.
Journal of immunological methods, 457, 73-77 (2018-02-25)
Standard cell culturing on plastic plates (two dimensional (2D) cultures) does not represent the actual microenvironment where cells reside in tissues. The three dimensional (3D) systems, composed of extracellular matrix and/or pure amino acids which form a scaffold for cells
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L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

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