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GE17-1235-01

Immobiline® Drystrip pH 3-10 NL, 18 cm

Cytiva 17-1235-01, pack of 12 strips

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.25

form

solid

feature

wetted part: no

packaging

pack of 12 strips

manufacturer/tradename

Cytiva 17-1235-01

color

Colorless

solubility

cold water: soluble (Insoluble in cold and hot water)

shipped in

dry ice

storage temp.

−20°C

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General description

Immobiline® DryStrip gels (IPG strips) are used for isoelectric focusing (IEF), run as the first dimension of 2-D electrophoresis or as a separate application.

Application

Immobiline® DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow-, medium-, and wide-range IPG strips available. The five narrow pH range (1 pH unit), 24-cm IPG strips deliver the highest resolution and Protein loading capacity available, which results in well separated spots with high Protein content after the second-dimension separation.

The pH 3–11 NL strip covers most Proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the Proteins evenly over the gel length to obtain maximal resolution.

Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of Protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan™ IPGphor 3 IEF system or Multiphor II flatbed system.

Features and Benefits

  • Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
  • Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
  • Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total Protein distribution.
  • For more detailed studies or when using greater Protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
  • Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
  • Use matching IPG buffers for optimized running conditions.
  • Use IPG strips directly after reswelling without further manipulation.
  • Barcoded strips for convenient sample and batch tracking.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ettan is a trademark of Cytiva
Immobiline is a registered trademark of Cytiva

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Troubleshooting Second-Dimension SDS-PAGE: Multiphor™ II Electrophoresis

This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.

Troubleshooting First-Dimension IEF: MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit

This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.

Protocols

Running a Multiphor II Protocol

This page shows protocols for running 7–24-cm Immobiline DryStrip gels on the Multiphor II Electrophoresis System.

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

Sample Application by Cup Loading

This protocol shows how to apply samples by cup loading for isoelectric focusing.

Preparing for IEF

This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.

See All

Related Content

Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels

This page shows how to apply equilibrated Immobiline DryStrip gels

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service