GE17-1279-03
rProtein A Sepharose™ Fast Flow
Cytiva 17-1279-03, pack of 200 mL
Synonym(s):
antibody affinity medium, antibody purification resin
About This Item
Recommended Products
ligand
recombinant protein A (E. coli)
packaging
pack of 200 mL
manufacturer/tradename
Cytiva 17-1279-03
storage condition
(20% Ehtanol)
matrix
4% cross-linked agarose
particle size
60-165 μm
average diameter
90 μm (d50v)
cleaning in place
2-11
working range
3-10
capacity
~50 mg binding capacity (human lgG/ml medium)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Related Categories
General description
rProtein A Sepharose™ 4 Fast Flow is based on the well established Sepharose™ Fast Flow platform and is used in many approved monoclonal antibody (MAb) processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.
rProtein A Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, rProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- High dynamic binding capacity due to oriented, single-point attachment of protein A
- Well-established Protein A medium used in many approved MAb processes
- No mammalian components involved during the manufacturing process
Storage and Stability
Analysis Note
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Articles
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Protocols
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
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