GE17-5280-01
nProtein A Sepharose™ 4 Fast Flow
Cytiva 17-5280-01, pack of 5 mL
Synonym(s):
Sepharose 4 Fast Flow
About This Item
Recommended Products
ligand
native protein A (S. aureus)
packaging
pack of 5 mL
manufacturer/tradename
Cytiva 17-5280-01
storage condition
(20% Ehtanol)
matrix
4% cross-linked agarose
average diameter
90 μm (d50v)
cleaning
2-10
working range
3-9
capacity
>30 mg binding capacity(human IgG/ml)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Related Categories
General description
nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to the well established Sepharose™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose™ 4 Fast Flow is manufactured without using animal-derived components.
nProtein A Sepharose™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.
As member of the BioProcess media range, nProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Replaces Protein A Sepharose™ 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
- Used in routine commercial production of monoclonal antibodies
- Free from animal-derived components.
Storage and Stability
Analysis Note
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Protocols
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