GE17-5279-02
Glutathione Seph High Performance
Cytiva 17-5279-02, pack of 100 mL
About This Item
Recommended Products
ligand
glutathione
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-5279-02
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
3-12
working range
3-12
capacity
>7 mg/mL binding capacity(medium)
Related Categories
General description
Application
Features and Benefits
- 34 μm bead size for improved efficiency and resolution
- Fast, one-step purification of GST-tagged Proteins and other glutathione S-trasferase or glutathione-dependent Proteins
- Mild conditions during the purification process preserves Protein antigenicity and function
Storage and Stability
Analysis Note
Legal Information
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Warning
hcodes
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Protocols
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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