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ligand
diethylaminoethyl
description
Ion Exchanger Type (value)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9269-82
parameter
44 psi
bed size
7.7 mm × 100 mm
bed volume
4.7 mL
column I.D.
7.7 mm
matrix
highly cross-linked agarose with dextran surface extender
average diameter
90 μm (d50v)
cleaning
2-14
working range
2-12
suitability
suitable for bioprocess medium
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General description
HiScreen Capto DEAE columns are prepacked with Capto DEAE ion exchange chromatography resins, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.
The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.
If necessary, two columns can easily be connected in series to give a bed height of 2NA cm.
Media prepacked in HiScreen Capto IEX columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.
If necessary, two columns can easily be connected in series to give a bed height of 2NA cm.
Media prepacked in HiScreen Capto IEX columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
Features and Benefits
- Capto DEAE is a weak anion exchange BioProcess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification.
- Prepacked, ready-to-use columns for convenient process development optimization.
- 1NA cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 2NA cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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