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I2276

Sigma-Aldrich

Interleukin-12 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

IL-12

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About This Item

MDL number:
MFCD00212975
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

200

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

0.01-0.05 ng/mL ED50

mol wt

glycoprotein 75 kDa

packaging

pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P29459,
P29460

storage temp.

−20°C

Gene Information

human ... IL12A(3592) , IL12B(3593)

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Biochem/physiol Actions

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Ruifen Sun et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(12), 9295-9301 (2015-06-25)
IL-12 is an antitumor cytokine with functions of inhibiting tumor growth, invasion, and metastasis, indicating that IL-12 is a promising candidate for cancer treatment. The aim of this study was to investigate the association of IL-12A rs568408, IL-12A rs2243115, and

Articles

Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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