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L8384

Sigma-Aldrich

Linoleic Acid-Albumin from bovine serum albumin

γ-irradiated, lyophilized powder, BioReagent, suitable for cell culture

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

biological source

bovine plasma

Quality Level

200

description

Contains 1.1-2.3 moles linoleic acid per mole BSA

sterility

γ-irradiated

product line

BioReagent

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mg endotoxin

solubility

water: 10 mg/mL, clear to hazy, colorless to light yellow

storage temp.

2-8°C

Related Categories

General description

Linoleic acid is an essential and highly consumed polyunsaturated fatty acid (PUFA). Albumin is a globular protein. Bovine serum albumin (BSA) is composed of a single polypeptide chain and cross-linked cysteine disulfide bridges. BSA is heart-shaped and is made up of three homologous domains I-III and each domain has two subdomains A and B.

Application

Linoleic Acid-Albumin from bovine serum albumin has been used: in cell culture and to study its effects on the human colonic adenocarcinoma cell line. It has also been used in the in vitro culture (IVC) of cow embryos/zygotes. The binding of linoleic acid to BSA improves its stability and solubility in culture media.

Biochem/physiol Actions

Linoleic acid plays a role in maintaining the transdermal water barrier in epidermis. Serum albumin constitutes about 52-62% of the total water-soluble fraction proteins in the blood plasma.

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wgk_germany

WGK 3

flash_point_f

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flash_point_c

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Certificates of Analysis (COA)

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Takemasa Hidaka et al.
Theriogenology, 113, 176-182 (2018-03-20)
Variations in embryo production between individual oocyte donors represent a serious problem in cattle production, when implementing ovum pick-up (OPU) and in vitro maturation (IVM) of oocytes. However, the precise cause of this problem is unknown. Here, we aimed to investigate

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Linoleic acid in Cell Culture

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