Skip to Content
MilliporeSigma
All Photos(1)

Documents

M061040

Sigma-Aldrich

Phosphate-ON Phosphoramidite

configured for MerMade

Synonym(s):

Phosphate-ON amidite

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C49H62N7O11P
Molecular Weight:
956.03
MDL number:
MFCD12912424
UNSPSC Code:
41116105
PubChem Substance ID:
329817815
NACRES:
NA.21

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

assay

≥98% (31P-NMR)
≥98.0% (reversed phase HPLC)

compatibility

configured for MerMade

storage temp.

−20°C

SMILES string

COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OC(=O)NCCCNC(=O)C[C@@H](CC#N)OP(OCCC#N)N(C(C)C)C(C)C)N3C=C(C)C(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5

InChI

1S/C49H62N7O11P/c1-33(2)56(34(3)4)68(64-28-11-24-50)67-41(23-25-51)29-44(57)52-26-12-27-53-48(60)66-42-30-45(55-31-35(5)46(58)54-47(55)59)65-43(42)32-63-49(36-13-9-8-10-14-36,37-15-19-39(61-6)20-16-37)38-17-21-40(62-7)22-18-38/h8-10,13-22,31,33-34,41-43,45H,11-12,23,26-30,32H2,1-7H3,(H,52,57)(H,53,60)(H,54,58,59)/t41-,42+,43-,45-,68?/m1/s1

InChI key

MCGIOYZUTKDSIL-NNUNARBDSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Phosphate-ON phosphoramidite is a chemical phosphorylation reagent that can be employed to introduce a phosphate group at the 5′ terminus of an oligonucleotide. Chemical phosphorylation is a cost-effective alternative to enzymatic phosphorylation methods, allowing the introduction of terminal phosphate groups in high yields, at any desired scale. We offer Phosphate-ON phosphoramidite in powder form, enabling the dissolution of the reagent in acetonitrile to be easily monitored.

Application

The application of Phosphate-ON Phosphoramidite is fully compatible with standard cleavage and deprotection procedures at the end of oligonucleotide synthesis. Phosphate-ON phosphoramidite comprises a thymidine nucleoside, which is removed from the oligonucleotide during the cleavage and deprotection process.

Other Notes

The key features of Phosphate-ON Phosphoramidite are as follows:
  • Powder format enables dissolution to be easily monitored and facilitatesproduct handling
  • Fully compatible with standard phosphoramidite reagents and synthesisconditions
  • Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotidecan be cleaved from the support and deprotected in concentratedammonia at 55 °C in only 2 hours
  • Comprises a DMT-group for trityl monitoring
  • Dissolves easily in acetonitrile to a 0.1 M solution
  • High coupling efficiency of ≥98.0% under standard DNA phosphoramiditecoupling conditions
  • Cleavage of both phosphate protective groups through a β-eliminationprocess

Legal Information

This product is covered under US Patent Number 7,276,598

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service