MAK023
Chloride Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
relevant disease(s)
pulmonary disorders
storage temp.
room temp
Related Categories
General description
Chloride is an essential anion needed for the proper functioning of many critical aspects of metabolism, including aiding in maintenance of the body′s acid-base balance. In Cystic Fibrosis, mutations to the CF transmembrane conductance regulator (CFTCR) gene result in altered sodium and chloride ion transport channels.
Application
Chloride Assay Kit has been used to measure chloride concentration spectrophotometrically and is used to measure chloride levels in blood plasma of toads Rhinella marina.
Suitability
Suitable for the quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water
Principle
The Chloride Assay kit provides a simple and direct procedure for measuring chloride in a variety of samples, including blood and urine. Chloride concentration is determined by a competition reaction between Hg2+ and Fe2+ for 2,4,6-Tris(2-pyridyl)-s-triazine(TPTZ). The preferred Hg-TPTZ complex exhibits no color. In the presence of chloride, Hg2+ forms HgCl2, which precipitates, allowing TPTZ to complex with Fe2+. The Fe-TPTZ complex results in a colorimetric (620 nm) product proportional to the chloride present.
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