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N0409

Sigma-Aldrich

Anti-Nitrotyrosine antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Nitrotyrosine Antibody, Nitrotyrosine Antibody - Anti-Nitrotyrosine antibody produced in rabbit

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About This Item

MDL number:
MFCD01322409
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

wide range

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain
indirect ELISA: 1:1,000 using nitrated-BSA
indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine
microarray: suitable
western blot: 1:1,000 using nitrated-BSA

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)

General description

Peroxinitrite (ONOO-), a potent oxidant linked to cellular oxidative damage, is an important member of the family of reactive oxygen and nitrogen species, rapidly generated in vivo from nitric oxide and superoxide anion. Nitrotyrosine is a product of tyrosine nitration, that is facilitated by reactive nitrogen species.

Specificity

Anti-Nitrotyrosine recognizes nitrated proteins and 3-nitro-L-tyrosine. It does not cross-react with L-tyrosine, ρ-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine and phospho-L-tyrosine BSA conjugates.
Reacts with peroxynitrite-nitrated proteins and 3-nitro-L-tyrosine. Does not crossreact with L-tyrosine, p-nitro-L-phenylalanine, 3-amino-L-tyrosine, 3-chloro-L-tyrosine, and phospho-L-tyrosine. Binding of the antibody is blocked by 3-nitrotyrosine.

Immunogen

nitrated KLH (Keyhole Limpet Hemocyanin).

Application

Anti-Nitrotyrosine antibody produced in rabbit has been used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • immunofluorescence immunocytochemistry
  • confocal microscopy
  • immunofluorescent staining

Biochem/physiol Actions

Peroxynitrite has been shown to promote the nitration of tyrosine residues in proteins, and oxidation of redox metal centers, DNA, lipids and cysteine and methionine residues in proteins, suggesting that it plays a major role in oxidative cellular damage. Peroxynitrite has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human disease, including neurological conditions such as Alzheimer′s disease, amyotrophic lateral sclerosis (ALS), in the pathogenesis of asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Purified by affinity chromatography on a column of 3-nitro-L-tyrosine.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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