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N8664

Sigma-Aldrich

Anti-NONO (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-NMT55, Anti-NRB54, Anti-Non-pou domain-containing octamer-binding protein, Anti-Nuclear RNA-binding protein, 54-KD, Anti-p54nrb

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells
western blot: 1-2 μg/mL using NIH-3T3 cell lysates

UniProt accession no.

Q15233

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NONO(4841)

Related Categories

General description

Non-POU domain containing octamer binding (NONO) is ubiquitously expressed and comprises of two tandem RNP-type RNA- recognition motifs and a putative helix-turn-helix domain followed by a highly charged region. It is enriched in paraspeckles, a nucleoplasmic compartment and relocalizes to cap structures at the nucleolar periphery when transcription is inhibited.
Non-POU domain containing octamer binding protein (NONO) is part of the Drosophila behavior/human splicing (DBHS) protein family. It has a molecular weight of 54kDa and the gene encoding it is localized on human chromosome Xq13.1.

Application

Anti-NONO (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Anti-NONO (N-terminal) antibody produced in rabbit has been used in immunoprecipitation.

Biochem/physiol Actions

NONO (also termed as p54nrb) is a nuclear protein implicated in numerous processes including transcription, pre-mRNA processing, nuclear retention of edited RNA and DNA relaxation. NONO was suspected to be involved in pre-mRNA splicing. Later it was found that NONO and PSF mediate different functions depending on their intracellular location.
Non-POU domain containing octamer binding protein (NONO) modulates transcription. Loss-of-function of the protein has been linked to human intellectual disability. The protein also has a role in synaptic transcription and regulation of the circadian clock. NONO also has a function in DNA damage response.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation
Nader A
PLoS ONE (2015)
A crystallographic study of human NONO (p54nrb): overcoming pathological problems with purification, data collection and noncrystallographic symmetry
Knott GJ, et al.
Acta crystallographica. Section D, Structural biology, 761-761 (2016)
Prefrontal cortex shotgun proteome analysis reveals
altered calcium homeostasis and immune system
imbalance in schizophrenia
Daniel Martins-de-Souza
European Journal of Anaesthesiology. Supplement (2009)
NONO regulates the intra-S-phase checkpoint in response to UV radiation.
Alfano L
Oncogene (2016)
Paraspeckles: a novel nuclear domain.
Fox AH, et al.
Current Biology, 12(1), 13-25 (2002)
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