Skip to Content
MilliporeSigma
All Photos(4)

Documents

SAB4200067

Sigma-Aldrich

Anti-PSPC1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-PSP1, Anti-Paraspeckle protein 1

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~45 kDa
antigen ~59 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells
indirect immunofluorescence: 1.25-2.5 μg/mL using paraformaldehyde fixed HEK-293T cells
western blot: 0.5-1 μg/mL using lysates of HEK-293T cells

UniProt accession no.

Q8WXF1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PSPC1(55269)
mouse ... Pspc1(66645)
rat ... Pspc1(305910)

General description

PSPC1 has two isoforms α and β.
Paraspeckle component 1 (PSPC1) is encoded by the gene mapped to human chromosome 13q12.11. The encoded protein is a member of DBHS (Drosophila Behavior Human Splicing) family of proteins.

Application

Anti-PSPC1 (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • ribonucleoprotein immunoprecipitation assay
  • immunofluorescence
  • immunocytochemistry

Biochem/physiol Actions

Paraspeckle component 1 (PSPC1) is involved in the regulation of nuclear receptors such as the progesterone receptor and the thyroid receptor and in a wide range of processes such as splicing and RNA metabolism.
Since paraspeckle component 1 (PSPC1) has lower background nucleoplasmic signal in various cell types compared to other members of DBHS (drosophila behavior human splicing) family of proteins, it is frequently used as a marker for paraspeckles. The encoded protein plays a vital role in regulation of transcription. Deletion of the gene is associated with the hindered psychomotor development, muscle hypotonia, unilateral microphtalmia with ptosis, congenital eye malformation and facial dysmorphic features.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during mouse spermiogenesis
Xu K, et al.
Biology of Reproduction, 90(6), 119-111 (2014)
Paraspeckles
Fox A H
Cold Spring Harbor Perspectives in Biology, 2 (2010)
The Hepatitis Delta Virus accumulation requires paraspeckle components and affects NEAT1 level and PSP1 localization
Beeharry Y, et al.
Scientific Reports, 8(1), 6031-6031 (2018)
PSF and p54nrb/NonO-multi-functional nuclear proteins
Shav-Tal Y and Zipori D
Febs Letters, 531(2), 109-114 (2002)
First-trimester euploid miscarriages analysed by array-CGH.
Viaggi CD
Journal of applied genetics, 54, 353-359 (2013)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service