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P1873

Sigma-Aldrich

Anti-Perilipin A/B antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-FPLD4, Anti-PERI, Anti-PLIN

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About This Item

MDL number:
MFCD07370429
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~62 kDa (also band at 46 kDa)

species reactivity

mouse

technique(s)

indirect immunofluorescence: 5-10 μg/mL using differentiated mouse NIH3T3-L1 cells
western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of differentiated mouse NIH3T3-L1 cells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PLIN(5346)
mouse ... Plin(103968)
rat ... Plin(25629)

General description

Perilipin is an intracellular neutral lipid storage droplet surface protein in white and brown fat adipocytes. It is also found in lower quantity coating droplets in steroidogenic-cells of the adrenal cortex, ovaries, and testicular Leydig cells, on the surface of smaller droplets containing cholesteryl esters.

Specificity

Rabbit polyclonal anti-Perilipin A/B antibody recognizes mouse Perilipin A/B by immunoblotting (46 kDa and ~62 kDa) and indirect immunofluorescence. Detection of the perilipin A and B bands by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding amino acid residues 6-17 of mouse and rat perilipin A/B with C-terminal added cysteine, conjugated to KLH. The corresponding human sequence differs by two residues.

Application

Anti-Perilipin A/B antibody produced in rabbit has been used in immunoblotting and immunofluorescence staining.
Rabbit polyclonal anti-Perilipin A/B antibody is used to tag Perilipin A/B for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Perilipin A/B in the regulation of triacylglycerol storage in adipocytes.

Biochem/physiol Actions

Perilipin is a gatekeeper protein that is involved in regulating triacylglycerol storage in adipocyte through the suppression of basal lipolysis apparently through protecting triacylglycerol against hydrolysis. Perilipin also enhances cAMP-dependent protein kinase (PKA)-stimulated lipolysis by hormone-sensitive lipase (HSL) and non-HSLs. Perilipin knockout mice exhibit reduced adipose tissue mass and resistance to diet induced obesity. Their lipid storage droplets are coated with adipose differentiation-related protein (ADRP, adipophilin), that is devoid of phosphorylation by PKA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Danielle R Rux et al.
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Proteomic analysis of murine testes lipid droplets
Wang W, et al.
Scientific reports, 5, 12070-12070 (2015)
Thomas V Lakeland et al.
American journal of physiology. Endocrinology and metabolism, 306(12), E1367-E1377 (2014-04-25)
Impaired coupling of adipose tissue expansion and vascularization is proposed to lead to adipocyte hypoxia and inflammation, which in turn contributes to systemic metabolic derangements. Pigment epithelium-derived factor (PEDF) is a powerful antiangiogenic factor that is secreted by adipocytes, elevated
The perilipin family of lipid droplet proteins: Gatekeepers of intracellular lipolysis
Sztalryd C and Brasaemle DL
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(10), 1221-1232 (2017)
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