Skip to Content
MilliporeSigma
All Photos(2)

Documents

PLA0148

Sigma-Aldrich

Rabbit anti-Histone H3 Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):

H3 histone family, H3/A, H3FA, H3a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, histone 1, histone H3/a, member A

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

human

technique(s)

immunohistochemistry: 1:200-1:1,000
immunoprecipitation (IP): 2-10 μg/mg
western blot: 1:2,000- 1:10,000

accession no.

NP_003520.1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

rabbit ... Histone H3(8350)

General description

Mammals contain variants of histone H3, that are, the replicative histones (H3.1 and H3.2) and the replacement histones (H3.3). Histone H3 is located on human chromosome 6p22.

Immunogen

The epitope recognized by PLA0148 maps to a region between residue 100 and the C-terminus (residue 135) of human Histone H3 using the numbering given in entry NP_003520.1 (GeneID 8350).

Biochem/physiol Actions

Histone proteins are the basic building blocks of chromatin. Histone H3 controls protein-protein interactions to induce binding of trans-acting factors that have a role in chromatin condensation. Histone H3 K27M mutations are observed in adult cerebellar high-grade gliomas.

Physical form

Tris-buffered Saline containing 0.1% BSA with 0.09% Sodium Azide

Other Notes

Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Still not finding the right product?  

Give our Product Selector Tool a try.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The program for processing newly synthesized histones H3. 1 and H4
Campos EL, et al.
Nature Structural Biology, 17(11), 1343-1343 (2010)
Signaling to chromatin through histone modifications.
Cheung P, et al.
Cell, 103(2), 263-271 (2000)
Histone H3 K27M mutations in adult cerebellar high-grade gliomas.
Nakata S, et al.
Brain Tumor Pathology, 34(3), 113-119 (2017)
Romain Pacaud et al.
Theranostics, 5(1), 12-22 (2015-01-02)
Histones post-translational modifications (PTMs) are crucial components of diverse processes that modulate chromatin. Among the histones PTMs, the histones phosphorylation appears such crucial since it plays a significant role into DNA repair structure, transcription and chromatin compaction during cell division
Athanasios Lourbopoulos et al.
Journal of molecular neuroscience : MN, 54(1), 78-91 (2014-03-01)
Thyroid hormones (TH) and receptors (TRs) may play an important role in the pathophysiology of acute cerebral ischemia. In the present study, we sought to determine whether serum triodothyronine (T3)/thyroxine (T4) and brain TRs (TRα1, TRβ1) might change after experimental
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service