Skip to Content
MilliporeSigma
All Photos(2)

Documents

PLA0221

Sigma-Aldrich

Rabbit anti-ORC1 Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):

HSORC1, ORC1L, PARC1, S. cerevisiae, homolog-like, origin recognition complex, origin recognition complex 1, replication control protein 1, subunit 1, subunit 1 (yeast homolog)-like, subunit 1 homolog, subunit 1 homolog (S. cerevisiae), subunit 1-like (yeast)

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

human

technique(s)

immunoprecipitation (IP): 2-5 μg/mg
western blot: 1:2,000- 1:10,000

accession no.

NP_004144.2

UniProt accession no.

Q13415

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

rabbit ... ORC1(4998)

General description

Origin recognition complex 1 (ORC1) is the largest subunit of ORC. The subunits are widely expressed in the human lung, bones, kidney, and uterus. The ORC1 gene is mapped to human chromosome 1p32.3.

Immunogen

The epitope recognized by PLA0221 maps to a region between residue 811 and 861 of human origin recognition complex subunit 1 using the numbering given in entry NP_004144.2 (GeneID 4998).

Application

Rabbit anti-ORC1 Antibody, Affinity Purified has been used in immunoblotting (1:2,000).

Biochem/physiol Actions

The origin recognition complex (ORC) plays a key role in DNA replication. ORC1 is degraded in the S phase and re-synthesized in the G1 phase of the cell cycle. ORC1 is responsible for cell proliferation, apoptosis, invasion, and migration. ORC1 regulates cyclin E1 (CCNE1) gene expression and is involved in genomic maintenance. Downregulation of ORC1 and cyclins cause Meier–Gorlin syndrome.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

ORC1 (origin recognition complex, subunit 1) is one of six subunits that make up the origin of recognition complex (ORC). The ORC serves as a scaffold for proteins involved in the initiation of DNA replication. Very recently, ORC1 has been found to perform a function independent of its role in DNA replication. In a screen using siRNAs for human ORC proteins to assess their roles in centrosome biology, it was found that ORC1 plays a key role in controlling centriole and centrosome copy number in human cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Xinxin Wang et al.
Cancer management and research, 10, 6971-6984 (2018-12-28)
Centromere protein U (CENPU) abnormally exhibits high expression in various types of human tumor tissues and participates in tumor progression; however, its expression pattern and biological function in lung cancer have not yet been elucidated. In the present study, we
Cari L Graber-Feesl et al.
Molecular cancer research : MCR, 17(8), 1687-1698 (2019-05-23)
Mitotic DNA synthesis is a recently discovered mechanism that resolves late replication intermediates, thereby supporting cell proliferation under replication stress. This unusual form of DNA synthesis occurs in the absence of RAD51 or BRCA2, which led to the identification of
Philippe Coulombe et al.
Nature communications, 10(1), 2426-2426 (2019-06-05)
DNA replication initiation is a two-step process. During the G1-phase of the cell cycle, the ORC complex, CDC6, CDT1, and MCM2-7 assemble at replication origins, forming pre-replicative complexes (pre-RCs). In S-phase, kinase activities allow fork establishment through (CDC45/MCM2-7/GINS) CMG-complex formation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service